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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH7B Polyclonal antibody (22300-1-AP)

    WB analysis using 22300-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22300-1-AP (MYH7B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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MYH7B Polyclonal antibody

MYH7B Polyclonal Antibody for WB, ELISA
Cat No. 22300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Antigen MLAA 21, KIAA1512, MHC14, MYH14, MYH7B, Myosin 7B, Slow A MYH14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22300-1-AP targets MYH7B in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 7B, cardiac muscle, beta
Calculated molecular weight 221 kDa
Observed molecular weight200-230 kDa
GenBank accession numberNM_020884
Gene Symbol MYH7B
Gene ID (NCBI) 57644
RRIDAB_3669394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA7E2Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin heavy chain 7b (MYH7b) is an ancient member of the myosin heavy chain motor protein family that is expressed in striated muscles. In mammalian cardiac muscle, MYH7b RNA is expressed along with two other myosin heavy chains, β-myosin heavy chain (β-MyHC) and α-myosin heavy chain (α-MyHC). However, unlike β-MyHC and α-MyHC, which are maintained in a careful balance at the protein level, the MYH7b locus does not produce a full-length protein in the heart due to a posttranscriptional exon-skipping mechanism that occurs in a tissue-specific manner (PMID: 33895132). MYH7b can be detected as about 200-230 kDa.

Protocols

Product Specific Protocols
WB protocol for MYH7B antibody 22300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols