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KD/KO Validated

MYH9 Polyclonal antibody

Name: MYH9 Polyclonal antibody
Brand: Proteintech
SKU: 11128-1-AP

MYH9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11128-1-AP

Synonyms

NMMHC-A, NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 11128-1-AP
Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11128-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 11128-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11128-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11128-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11128-1-AP
IP result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human lung cancer using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11128-1-AP (MYH9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11128-1-AP (MYH9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11128-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 11128-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11128-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 11128-1-AP
Immunofluorescent analysis of HepG2 cells, using MYH9 antibody 11128-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of HepG2 using 11128-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYH9 Polyclonal antibody (11128-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11128-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, mouse kidney tissue, mouse colon tissue, human brain tissue, U2OS cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse small intestine tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 33 publications below
IHC	See 9 publications below
IF	See 18 publications below
IP	See 5 publications below
CoIP	See 4 publications below

Product Information

11128-1-AP targets MYH9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYH9 fusion protein Ag1612 相同性解析による交差性が予測される生物種
Full Name	myosin, heavy chain 9, non-muscle
Calculated molecular weight	227 kDa
Observed molecular weight	224 kDa
GenBank accession number	BC011915
Gene symbol	MYH9
Gene ID (NCBI)	4627
RRID	AB_2147294
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
WB protocol for MYH9 antibody 11128-1-AP	Download protocol
IHC protocol for MYH9 antibody 11128-1-AP	Download protocol
IF protocol for MYH9 antibody 11128-1-AP	Download protocol
IP protocol for MYH9 antibody 11128-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Acta Pharm Sin B Ginsenoside Rg1 ameliorates blood-brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release. Authors - Kefeng Zhai View Article
human	WB	Sci Adv Suppression of non-muscle myosin II boosts T cell cytotoxicity against tumors Authors - Yingyun Yang View Article
human	WB	J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK Authors - Li Cui View Article
human	WB,IF	Cell Death Differ Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling Authors - Shunfeng Hu KD Validated View Article
human,mouse	WB,IHC,IF	Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells Authors - Yingqi Liu View Article
mouse	IF,CoIP,WB	Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. Authors - Gao-Shan Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 11128-1-AP

WB analysis of mouse kidney using 11128-1-AP

WB analysis of mouse colon using 11128-1-AP

WB analysis of human brain using 11128-1-AP

WB analysis of mouse brain using 11128-1-AP

IP experiment of mouse brain using 11128-1-AP

IHC staining of human lung cancer using 11128-1-AP

IHC staining of human lung cancer using 11128-1-AP

IHC staining of human colon using 11128-1-AP

IHC staining of human kidney using 11128-1-AP

IHC staining of mouse kidney using 11128-1-AP

IHC staining of mouse kidney using 11128-1-AP

IF Staining of mouse small intestine using 11128-1-AP

IF Staining of HepG2 using 11128-1-AP

IF Staining of HepG2 using 11128-1-AP

FC experiment of HepG2 using 11128-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ginsenoside Rg1 ameliorates blood-brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release.

Suppression of non-muscle myosin II boosts T cell cytotoxicity against tumors

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

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