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  • Featured Product
  • KD/KO Validated

MYH9 Monoclonal antibody, PBS Only

MYH9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D9D2

Cat no : 60233-1-PBS

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of human kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of pig spleen using 60233-1-Ig (same clone as 60233-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig pancreas tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of pig pancreas using 60233-1-Ig (same clone as 60233-1-PBS)

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Monoclonal antibody (60233-1-Ig)

    IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYH9 Monoclonal antibody (60233-1-Ig)

    IF Staining of U2OS using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60233-1-PBS targets MYH9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYH9 fusion protein Ag1612 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight 230 kDa, 160 kDa
GenBank accession numberBC011915
Gene symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).