• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of human kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of pig spleen using 60233-1-Ig (same clone as 60233-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig pancreas tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    WB analysis of pig pancreas using 60233-1-Ig (same clone as 60233-1-PBS)

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MYH9 Monoclonal antibody (60233-1-Ig)

    IHC staining of human skin cancer using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Monoclonal antibody (60233-1-Ig)

    IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYH9 Monoclonal antibody (60233-1-Ig)

    IF Staining of U2OS using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

MYH9 Monoclonal antibody, PBS Only

MYH9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60233-1-PBS
Clone No.5D9D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【全製品対象☆】小さなギフトがついてくる！春の特典キャンペーン開催中

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60233-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYH9 fusion protein Ag1612 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight 230 kDa, 160 kDa
GenBank accession numberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
RRIDAB_2881357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).