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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYH9 Recombinant antibody

MYH9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E1

Cat no : 81204-1-RR

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH9 Recombinant antibody (81204-1-RR)

    WB analysis using 81204-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81204-1-RR (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MYH9 Recombinant antibody (81204-1-RR)

    IHC staining of human colon cancer using 81204-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MYH9 Recombinant antibody (81204-1-RR)

    IHC staining of rat kidney using 81204-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Recombinant antibody (81204-1-RR)

    IF Staining of HepG2 using 81204-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (81204-1-RR, Clone: 3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81204-1-RR

    Affinity and Kinetic Characterization of 81204-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81204-1-RR against Human MYH9 were performed. The affinity constant is 0.297 nM.

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Tested Applications

Positive WB detected inU-251 cells, U-87 MG cells, HeLa cells, A431 cells, U2OS cells, human placenta tissue, rat kidney tissue
Positive IHC detected inrat kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4950-1:19800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81204-1-RR targets MYH9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYH9 fusion protein Ag1612 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight224-227 kDa
GenBank accession numberBC011915
Gene symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
WB protocol for MYH9 antibody 81204-1-RRDownload protocol
IHC protocol for MYH9 antibody 81204-1-RRDownload protocol
IF protocol for MYH9 antibody 81204-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols