Validation Data Gallery

Filter: AllWBIHC

WB analysis of human skeletal muscle using 15814-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15814-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15814-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15814-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 15814-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of rat skeletal muscle using 15814-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skeletal muscle using 15814-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 15814-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skeletal muscle using 15814-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15814-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human skeletal muscle tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse skin tissue
Positive IHC detected in	rat skeletal muscle tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

15814-1-AP targets MYL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8594 Product name: Recombinant human MYL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC005318 Sequence: MSFSADQIAEFKEAFLLFDRTGDSKITLSQVGDVLRALGTNPTNAEVRKVLGNPSNEELNAKKIEFEQFLPMMQAISNNKDQATYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALMAGQEDSNGCINYEAFVKHIMSI 相同性解析による交差性が予測される生物種
Full Name	myosin, light chain 1, alkali; skeletal, fast
Calculated molecular weight	150aa,17 kDa; 194aa,22 kDa
Observed molecular weight	14-17 kDa, 22-25 kDa
GenBank accession number	BC005318
Gene Symbol	MYL1
Gene ID (NCBI)	4632
RRID	AB_2266747
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05976
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for MYL1 antibody 15814-1-AP	Download protocol
WB protocol for MYL1 antibody 15814-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nature SAR1B senses leucine levels to regulate mTORC1 signalling. Authors - Jie Chen View Article
mouse	WB	Cell Prolif KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Authors - Jing Hao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human skeletal muscle using 15814-1-AP

WB analysis of mouse skeletal muscle using 15814-1-AP

WB analysis of mouse heart using 15814-1-AP

WB analysis of human heart using 15814-1-AP

WB analysis of mouse skin using 15814-1-AP

IHC staining of rat skeletal muscle using 15814-1-AP

IHC staining of rat skeletal muscle using 15814-1-AP

IHC staining of mouse skeletal muscle using 15814-1-AP

IHC staining of rat skeletal muscle using 15814-1-AP

MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

SAR1B senses leucine levels to regulate mTORC1 signalling.

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.