MYL12A Polyclonal antibody

Name: MYL12A Polyclonal antibody
Brand: Proteintech
SKU: 16287-1-AP

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16287-1-AP

Synonyms

MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of NIH/3T3 using 16287-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16287-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16287-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16287-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 16287-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse bladder using 16287-1-AP
mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16287-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16287-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 16287-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16287-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 16287-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 16287-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 16287-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, human skeletal muscle tissue, mouse bladder tissue, Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue
Positive IHC detected in	human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYL12A fusion protein Ag9413 相同性解析による交差性が予測される生物種
Full Name	myosin, light chain 12A, regulatory, non-sarcomeric
Calculated molecular weight	171 aa, 20 kDa
Observed molecular weight	19-20 kDa
GenBank accession number	BC016372
Gene symbol	MYL12A
Gene ID (NCBI)	10627
RRID	AB_2878238
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MYL12A antibody 16287-1-AP	Download protocol
IHC protocol for MYL12A antibody 16287-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	Biochem J Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli. Authors - Shcherbakova Olga V OV View Article
mouse	WB	Front Physiol Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction. Authors - Jie Sun View Article
human	IHC	Biofizika [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium]. Authors - Marchenko A V AV View Article
mouse	WB	EMBO J Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier Authors - Yuanji Dai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYL12A Polyclonal antibody

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 16287-1-AP

WB analysis of HeLa using 16287-1-AP

WB analysis of HEK-293 using 16287-1-AP

WB analysis of mouse heart using 16287-1-AP

WB analysis of human skeletal muscle using 16287-1-AP

WB analysis of mouse bladder using 16287-1-AP

WB analysis of NIH/3T3 using 16287-1-AP

WB analysis of Jurkat using 16287-1-AP

IHC staining of human heart using 16287-1-AP

IHC staining of human heart using 16287-1-AP

IHC staining of human skeletal muscle using 16287-1-AP

IHC staining of human skeletal muscle using 16287-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.

Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.

[Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].

Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier