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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

    Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    WB analysis of mouse heart using 66286-1-Ig (same clone as 66286-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50642-3

    Sandwich ELISA standard curve of MP50642-3

    Sandwich ELISA standard curve of MP50642-3, Human MYL3 Monoclonal Matched Antibody Pair - PBS only. 66286-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24535. 66286-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP50642-3

    Sample test of MP50642-3

    The mean MYL3 concentration was determined to be 23.06 ng/mL in mouse heart tissue extract based on a 6.9 mg/mL extract load and 3.2 ng/mL in mouse skeletal muscle tissue extract based on a 1.7 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYL3 Monoclonal antibody (66286-1-Ig)

    IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3, MYL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66286-1-PBS. Detection antibody: 66286-5-PBS. Standard:Ag24535. Range: 0.098-6.25 ng/mL.

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MYL3 Monoclonal antibody, PBS Only (Capture)

MYL3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66286-1-PBS
Clone No.2E4B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66286-1-PBS targets MYL3 as part of a matched antibody pair:

MP50642-3: 66286-1-PBS capture and 66286-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL3 fusion protein Ag24535 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC009790
Gene Symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2881669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08590
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)