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- KD/KO Validated
MYL4 Monoclonal antibody
MYL4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2F5C1
Cat no : 67533-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig heart tissue, K-562 cells, Mo7e cells, TF-1 cells, rat heart tissues, mouse heart tissues |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
Product Information
67533-1-Ig targets MYL4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MYL4 fusion protein Ag19244 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, light chain 4, alkali; atrial, embryonic |
| Calculated molecular weight | 197 aa, 22 kDa |
| Observed molecular weight | 22-25 kDa |
| GenBank accession number | BC030228 |
| Gene symbol | MYL4 |
| Gene ID (NCBI) | 4635 |
| RRID | AB_2882752 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| IHC protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| IF protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| FC protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes. | ||
J Proteome Res Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts | ||
Genes (Basel) Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs
| ||
Gene Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size | ||
Cell Biosci Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers | ||
Front Med Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart |
