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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MYL4 Monoclonal antibody

MYL4 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F5C1

Cat no : CL488-67533

Synonyms

ALC1, AMLC, GT1, MLC1, MYL4, Myosin light chain 4, PRO1957

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 488-conjugated MYL4 Monoclonal anti (CL488-67533)

    IF Staining of mouse heart using CL488-67533

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 MYL4 antibody (CL488-67533, Clone: 2F5C1 ) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67533 targets MYL4 in IF-P applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MYL4 fusion protein Ag19244 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 4, alkali; atrial, embryonic
Calculated molecular weight 197 aa, 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC030228
Gene symbol MYL4
Gene ID (NCBI) 4635
RRIDAB_2919493
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYL4 antibody CL488-67533Download protocol
Standard Protocols
Click here to view our Standard Protocols