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  • Featured Product
  • KD/KO Validated

MYL6 Monoclonal antibody, PBS Only

MYL6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G4C9

Cat no : 68142-1-PBS

Synonyms

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL6 Monoclonal antibody (68142-1-Ig)

    WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MYL6 Monoclonal antibody (68142-1-Ig)

    WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of rat heart using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of mouse skeletal muscle using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of rat skeletal muscle using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of human kidney using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYL6 Monoclonal antibody (68142-1-Ig)

    IHC staining of mouse heart using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYL6 Monoclonal antibody (68142-1-Ig)

    IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68142-1-PBS targets MYL6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL6 fusion protein Ag13091 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 6, alkali, smooth muscle and non-muscle
Calculated molecular weight 17 kDa
Observed molecular weight17-25 kDa
GenBank accession numberBC006781
Gene symbol MYL6
Gene ID (NCBI) 4637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).