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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYL6B Polyclonal antibody, PBS Only

MYL6B Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16963-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL6B Polyclonal antibody (16963-1-AP)

    WB analysis using 16963-1-AP (same clone as 16963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16963-1-AP (MYL6B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYL6B Polyclonal antibody (16963-1-AP)

    IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYL6B Polyclonal antibody (16963-1-AP)

    IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16963-1-PBS targets MYL6B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL6B fusion protein Ag10683 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 6B, alkali, smooth muscle and non-muscle
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC012425
Gene symbol MYL6B
Gene ID (NCBI) 140465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240).