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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL7 Recombinant antibody (81570-1-RR)

    WB analysis using 81570-1-RR (same clone as 81570-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81570-1-RR (MYL7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81570-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYL7 Recombinant antibody (81570-1-RR)

    IHC staining of mouse heart using 81570-1-RR (same clone as 81570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81570-1-RR (MYL7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81570-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81570-1-PBS

    Affinity and Kinetic Characterization of 81570-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81570-1-RR against Human MYL7 were performed. The affinity constant is 0.25 nM.

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MYL7 Recombinant antibody, PBS Only

MYL7 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81570-1-PBS
Clone No.2C5

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, Indirect ELISA

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81570-1-PBS targets MYL7 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYL7 fusion protein Ag11058 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 7, regulatory
Calculated molecular weight 175 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC027915
Gene Symbol MYL7
Gene ID (NCBI) 58498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01449
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.