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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYLK2 Polyclonal antibody

MYLK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21173-1-AP

Synonyms

KMLC, MLCK, MLCK2, MYLK2, myosin light chain kinase 2, skMLCK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of human skeletal muscle using 21173-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of Jurkat using 21173-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, Jurkat cells, A549 cells, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21173-1-AP targets MYLK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK2 fusion protein Ag15424 相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase 2
Calculated molecular weight 596 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC069627
Gene symbol MYLK2
Gene ID (NCBI) 85366
RRIDAB_10733123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).

Protocols

Product Specific Protocols
WB protocol for MYLK2 antibody 21173-1-APDownload protocol
IHC protocol for MYLK2 antibody 21173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.

Authors - Anteneh Getachew
View Article
humanWB

Microbiol Spectr

Berberine Depresses Inflammation and Adjusts Smooth Muscle to Ameliorate Ulcerative Colitis of Cats by Regulating Gut Microbiota

Authors - Xueying Li
View Article