Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, rat heart tissue |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
21527-1-AP targets MYLK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYLK3 fusion protein Ag16137 相同性解析による交差性が予測される生物種 |
| Full Name | myosin light chain kinase 3 |
| Calculated molecular weight | 819 aa, 88 kDa |
| Observed molecular weight | 85-100 kDa |
| GenBank accession number | BC109097 |
| Gene Symbol | MYLK3 |
| Gene ID (NCBI) | 91807 |
| RRID | AB_10734432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q32MK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYLK3 antibody 21527-1-AP | Download protocol |
| IHC protocol for MYLK3 antibody 21527-1-AP | Download protocol |
| IF protocol for MYLK3 antibody 21527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. | ||
Front Pharmacol GYY4137, as a slow-releasing H2S donor, ameliorates sodium deoxycholate-induced chronic intestinal barrier injury and gut microbiota dysbiosis | ||
Cell Commun Signal GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment | ||