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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYLK3 Polyclonal antibody

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21527-1-AP

Synonyms

Myosin light chain kinase 3, MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MYLK3 Polyclonal antibody (21527-1-AP)

    WB analysis using 21527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    WB analysis of human heart using 21527-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human colon using 21527-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human colon using 21527-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human heart using 21527-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK3 Polyclonal antibody (21527-1-AP)

    IHC staining of human heart using 21527-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYLK3 Polyclonal antibody (21527-1-AP)

    IF Staining of HepG2 using 21527-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYLK3 Polyclonal antibody (21527-1-AP)

    IF Staining of HepG2 using 21527-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, rat heart tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21527-1-AP targets MYLK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK3 fusion protein Ag16137 相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase 3
Calculated molecular weight 819 aa, 88 kDa
Observed molecular weight 85-100 kDa
GenBank accession numberBC109097
Gene symbol MYLK3
Gene ID (NCBI) 91807
RRIDAB_10734432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).

Protocols

Product Specific Protocols
WB protocol for MYLK3 antibody 21527-1-APDownload protocol
IHC protocol for MYLK3 antibody 21527-1-APDownload protocol
IF protocol for MYLK3 antibody 21527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
View Article
human,mouseIHC,WB,IF

Cell Commun Signal

GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment

Authors - Jia Mao
View Article
mouseWB

Nat Commun

Capture of RNA-binding proteins across mouse tissues using HARD-AP

Authors - Yijia Ren
View Article