Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, human heart tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83673-3-RR targets MYLK3 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MYLK3 fusion protein Ag16137 相同性解析による交差性が予測される生物種 |
Full Name | myosin light chain kinase 3 |
Calculated molecular weight | 819 aa, 88 kDa |
Observed molecular weight | 95 kDa |
GenBank accession number | BC109097 |
Gene Symbol | MYLK3 |
Gene ID (NCBI) | 91807 |
RRID | AB_3671278 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q32MK0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).
Protocols
Product Specific Protocols | |
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WB protocol for MYLK3 antibody 83673-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |