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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYLK4 Polyclonal antibody (24309-1-AP)

    WB analysis using 24309-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MYLK4 Polyclonal antibody (24309-1-AP)

    WB analysis of HEK-293 using 24309-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using MYLK4 Polyclonal antibody (24309-1-AP)

    WB analysis of L02 using 24309-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MYLK4 Polyclonal antibody (24309-1-AP)

    IP experiment of HEK-293 using 24309-1-AP

    IP result of anti-MYLK4 (IP:24309-1-AP, 4ug; Detection:24309-1-AP 1:500) with HEK-293 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK4 Polyclonal antibody (24309-1-AP)

    IHC staining of human heart using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLK4 Polyclonal antibody (24309-1-AP)

    IHC staining of human heart using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MYLK4 Polyclonal antibody (24309-1-AP)

    IHC staining of human tonsillitis using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MYLK4 Polyclonal antibody (24309-1-AP)

    IHC staining of human tonsillitis using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

MYLK4 Polyclonal antibody

MYLK4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24309-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Synonyms

MLCK4, MYLK4, SgK085, Sugen kinase 85

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, L02 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24309-1-AP targets MYLK4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK4 fusion protein Ag19314 相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase family, member 4
Calculated molecular weight 388 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC132831
Gene Symbol MYLK4
Gene ID (NCBI) 340156
RRIDAB_2877632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MYLK4 antibody 24309-1-APDownload protocol
IHC protocol for MYLK4 antibody 24309-1-APDownload protocol
IP protocol for MYLK4 antibody 24309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.

Authors - Mengkai Yang
  • KD Validated
View Article
IF

bioRxiv

GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis

Authors - Francesca Gobbo
View Article