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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYLPF Polyclonal antibody

MYLPF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16052-1-AP

Synonyms

HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16052-1-AP targets MYLPF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLPF fusion protein Ag9013 相同性解析による交差性が予測される生物種
Full Name myosin light chain, phosphorylatable, fast skeletal muscle
Calculated molecular weight 169 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC012571
Gene symbol MYLPF
Gene ID (NCBI) 29895
RRIDAB_2148036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYLPF antibody 16052-1-APDownload protocol
IHC protocol for MYLPF antibody 16052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model.

Authors - Ryohei Oya
View Article
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
View Article