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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYNN Polyclonal antibody

MYNN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13369-1-AP

Synonyms

MYNN, myoneurin, OSZF, SBBIZ1, ZBTB31

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis using 13369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis of mouse brain using 13369-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inCOLO 320 cells, Caco-2 cells, mouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13369-1-AP targets MYNN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYNN fusion protein Ag4188 相同性解析による交差性が予測される生物種
Full Name myoneurin
Calculated molecular weight 610 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC033620
Gene symbol MYNN
Gene ID (NCBI) 55892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myoneurin (MYNN) gene locates on 3q26.2 and encodes a member of the BTB/POZ and zinc finger (ZF) domain-containing protein family that is involved in the control of gene expression. MYNN is widely expressed in various tissue types in mammals. MYNN has 4 isoforms with the molecular mass of 10, 57, 65-69 kDa. (PMID: 30120764)

Protocols

Product Specific Protocols
WB protocol for MYNN antibody 13369-1-APDownload protocol
IHC protocol for MYNN antibody 13369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols