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  • Featured Product
  • KD/KO Validated

MYO19 Polyclonal antibody

MYO19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23906-1-AP

Synonyms

MYO19, MYOHD1, myosin XIX

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of A549 using 23906-1-AP

    WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of HeLa using 23906-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of COS-7 using 23906-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of A549 using 23906-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of human kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of rat kidney using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of rat testis using 23906-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    IF Staining of A549 using 23906-1-AP

    Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, COS-7 cells
Positive IHC detected inhuman kidney tissue, rat kidney tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23906-1-AP targets MYO19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey, rat samples.

Tested Reactivity human, monkey, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO19 fusion protein Ag20995 相同性解析による交差性が予測される生物種
Full Name myosin XIX
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC008900
Gene symbol MYO19
Gene ID (NCBI) 80179
RRIDAB_2879357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.

Protocols

Product Specific Protocols
WB protocol for MYO19 antibody 23906-1-APDownload protocol
IHC protocol for MYO19 antibody 23906-1-APDownload protocol
IF protocol for MYO19 antibody 23906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols