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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYO1G Polyclonal antibody, PBS Only

MYO1G Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25772-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO1G Polyclonal antibody (25772-1-AP)

    WB analysis using 25772-1-AP (same clone as 25772-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Polyclonal antibody (25772-1-AP)

    IF Staining of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25772-1-PBS targets MYO1G in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO1G fusion protein Ag22696 相同性解析による交差性が予測される生物種
Full Name myosin IG
Calculated molecular weight 1018 aa, 116 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC113544
Gene symbol MYO1G
Gene ID (NCBI) 64005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.