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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYO3A Polyclonal antibody

MYO3A Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28562-1-AP

Synonyms

DFNB30, MYO3A, myosin IIIA

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse eye tissue using MYO3A Polyclonal antibody (28562-1-AP)

    IHC staining of mouse eye using 28562-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using MYO3A Polyclonal antibody (28562-1-AP)

    IHC staining of mouse eye using 28562-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using MYO3A Polyclonal antibody (28562-1-AP)

    IF Staining of mouse eye using 28562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using MYO3A Polyclonal antibody (28562-1-AP)

    IF Staining of mouse eye using 28562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28562-1-AP targets MYO3A in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO3A fusion protein Ag29335 相同性解析による交差性が予測される生物種
Full Name myosin IIIA
Calculated molecular weight 186 kDa
Observed molecular weight190 kda
GenBank accession numberNM_017433
Gene symbol MYO3A
Gene ID (NCBI) 53904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MYO3A antibody 28562-1-APDownload protocol
IF protocol for MYO3A antibody 28562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols