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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYO5A Polyclonal antibody

MYO5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55318-1-AP

Synonyms

GS1, MYH12, MYO5A, Myosin 12, Myosin heavy chain 12, Myosin Va, Myoxin, MYR12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO5A Polyclonal antibody (55318-1-AP)

    WB analysis using 55318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55318-1-AP (MYO5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYO5A Polyclonal antibody (55318-1-AP)

    IHC staining of mouse brain using 55318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYO5A Polyclonal antibody (55318-1-AP)

    IHC staining of mouse brain using 55318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55318-1-AP targets MYO5A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin VA (heavy chain 12, myoxin)
Calculated molecular weight 215 kDa
Observed molecular weight210-220 kDa
GenBank accession numberNM_000259
Gene symbol MYO5A
Gene ID (NCBI) 4644
RRIDAB_2918356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYO5A antibody 55318-1-APDownload protocol
IHC protocol for MYO5A antibody 55318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols