• Featured Product
  • KD/KO Validated

MYO9B Polyclonal antibody

MYO9B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 12432-1-AP

Synonyms

Unconventional myosin-IXb, Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein


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Tested Applications

Positive WB detected inmouse thymus tissue, HL-60 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO9B fusion protein Ag3108 相同性解析による交差性が予測される生物種
Full Name myosin IXB
Calculated molecular weight 2023 aa, 223 kDa
Observed molecular weight 230-250 kDa
GenBank accession numberBC018108
Gene symbol MYO9B
Gene ID (NCBI) 4650
RRIDAB_2148635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYO9B antibody 12432-1-APDownload protocol
IHC protocol for MYO9B antibody 12432-1-APDownload protocol
IP protocol for MYO9B antibody 12432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset

Authors - Jing Zhang
humanWB

J Thromb Haemost

The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets.

Authors - Shane Comer
humanWB,IF

Cell Rep

Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.

Authors - Katarzyna A Makowska
  • KD Validated
mouseIF

J Bone Miner Res

The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.

Authors - Brooke K McMichael
  • KO Validated
mouseWB

J Immunol

TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.

Authors - Feng-Shuang Yi
humanWB

J Mol Biol

Non-canonical Myo9b-RhoGAP accelerates RhoA GTP hydrolysis by a dual-arginine-finger mechanism.

Authors - Fengshuang Yi
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sammy (Verified Customer) (11-20-2023)

Lots of speckles, but also observed at focal adhesions where it is reported in the literature.

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: 1 in 200
  • Cell Tissue Type: HUVEC
MYO9B Antibody Immunofluorescence,Flow Cytometry validation (1 in 200 dilution) in HUVEC (Cat no:12432-1-AP)