- Featured Product
- KD/KO Validated
MYO9B Polyclonal antibody
MYO9B Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 12432-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse thymus tissue, HL-60 cells, Jurkat cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 7 publications below |
IF | See 5 publications below |
Product Information
12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MYO9B fusion protein Ag3108 相同性解析による交差性が予測される生物種 |
Full Name | myosin IXB |
Calculated molecular weight | 2023 aa, 223 kDa |
Observed molecular weight | 230-250 kDa |
GenBank accession number | BC018108 |
Gene symbol | MYO9B |
Gene ID (NCBI) | 4650 |
RRID | AB_2148635 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MYO9B antibody 12432-1-AP | Download protocol |
IHC protocol for MYO9B antibody 12432-1-AP | Download protocol |
IP protocol for MYO9B antibody 12432-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset | ||
J Thromb Haemost The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets. | ||
Cell Rep Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.
| ||
J Bone Miner Res The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.
| ||
J Immunol TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response. | ||
J Mol Biol Non-canonical Myo9b-RhoGAP accelerates RhoA GTP hydrolysis by a dual-arginine-finger mechanism.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sammy (Verified Customer) (11-20-2023) | Lots of speckles, but also observed at focal adhesions where it is reported in the literature.
|