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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO9B Recombinant antibody (84090-4-RR)

    WB analysis using 84090-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84090-4-RR (MYO9B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using MYO9B Recombinant antibody (84090-4-RR)

    FC experiment of A431 using 84090-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MYO9B Recombinant antibody (84090-4-RR, Clone:241163D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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MYO9B Recombinant antibody

MYO9B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84090-4-RR
Clone No.241163D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, HL-60 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84090-4-RR targets MYO9B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYO9B fusion protein Ag33349 相同性解析による交差性が予測される生物種
Full Name myosin IXB
Calculated molecular weight 2023 aa, 223 kDa
Observed molecular weight 230-250 kDa
GenBank accession numberBC018108
Gene Symbol MYO9B
Gene ID (NCBI) 4650
RRIDAB_3671656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYO9B antibody 84090-4-RRDownload protocol
FC protocol for MYO9B antibody 84090-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols