Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, human stomach tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
14238-1-AP targets Myocilin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Myocilin fusion protein Ag5492 相同性解析による交差性が予測される生物種 |
| Full Name | myocilin, trabecular meshwork inducible glucocorticoid response |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC029261 |
| Gene Symbol | Myocilin |
| Gene ID (NCBI) | 4653 |
| RRID | AB_2148652 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myocilin antibody 14238-1-AP | Download protocol |
| IP protocol for Myocilin antibody 14238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann Transl Med Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma. | ||
Int J Ophthalmol Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork | ||
J Ocul Pharmacol Ther Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function | ||
Int J Mol Sci The Delayed Turnover of Proteasome Processing of Myocilin upon Dexamethasone Stimulation Introduces the Profiling of Trabecular Meshwork Cells' Ubiquitylome | ||
Discov Oncol Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining | ||