Myocilin Polyclonal antibody
Myocilin Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 14238-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human heart tissue, human stomach tissue, mouse skeletal muscle tissue |
Positive IP detected in | mouse skeletal muscle tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
14238-1-AP targets Myocilin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Myocilin fusion protein Ag5492 相同性解析による交差性が予測される生物種 |
Full Name | myocilin, trabecular meshwork inducible glucocorticoid response |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC029261 |
Gene symbol | Myocilin |
Gene ID (NCBI) | 4653 |
RRID | AB_2148652 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).
Protocols
Product Specific Protocols | |
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WB protocol for Myocilin antibody 14238-1-AP | Download protocol |
IP protocol for Myocilin antibody 14238-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Ann Transl Med Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma. | ||
Int J Ophthalmol Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork | ||
J Ocul Pharmacol Ther Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function |