Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 18943-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 18943-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 18943-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 18943-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 18943-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 18943-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse embryo using 18943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 18943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 18943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18943-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYOD1 antibody (18943-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

MYOD1 Polyclonal antibody

MYOD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18943-1-AP

Publications(117)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

MYO D1, Myf-3, MYF3, Myf 3, Class C basic helix-loop-helix protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (117)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected in	mouse embryo tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 91 publications below
IHC	See 13 publications below
IF	See 30 publications below
ChIP	See 1 publications below

Product Information

18943-1-AP targets MYOD1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYOD1 fusion protein Ag13512 相同性解析による交差性が予測される生物種
Full Name	myogenic differentiation 1
Calculated molecular weight	320 aa, 35 kDa
Observed molecular weight	35-45 kDa
GenBank accession number	BC064493
Gene Symbol	MYOD1
Gene ID (NCBI)	4654
RRID	AB_10603467
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15172
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Protocols

Product Specific Protocols
WB protocol for MYOD1 antibody 18943-1-AP	Download protocol
IHC protocol for MYOD1 antibody 18943-1-AP	Download protocol
IF protocol for MYOD1 antibody 18943-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature A plant-derived natural photosynthetic system for improving cell anabolism Authors - Pengfei Chen View Article
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
	WB	Nature SAR1B senses leucine levels to regulate mTORC1 signalling. Authors - Jie Chen View Article
rat	IF	Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction Authors - Yuzhi Sun View Article
mouse	WB	Nat Commun The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle. Authors - Giovanna Rigillo View Article
mouse	WB	J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. Authors - Junyu Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18943-1-AP

WB analysis using 18943-1-AP

WB analysis of mouse skeletal muscle using 18943-1-AP

WB analysis of human heart using 18943-1-AP

WB analysis of rat heart using 18943-1-AP

WB analysis of mouse heart using 18943-1-AP

IHC staining of mouse embryo using 18943-1-AP

IHC staining of mouse embryo using 18943-1-AP

IHC staining of mouse embryo using 18943-1-AP

IF Staining of HeLa using 18943-1-AP

MYOD1 Polyclonal antibody

MYOD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A plant-derived natural photosynthetic system for improving cell anabolism

Divergent transcriptional regulation of astrocyte reactivity across disorders.

SAR1B senses leucine levels to regulate mTORC1 signalling.

Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction

The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.

circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.