Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 91 publications below |
| IHC | See 13 publications below |
| IF | See 30 publications below |
| ChIP | See 1 publications below |
Product Information
18943-1-AP targets MYOD1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYOD1 fusion protein Ag13512 相同性解析による交差性が予測される生物種 |
| Full Name | myogenic differentiation 1 |
| Calculated molecular weight | 320 aa, 35 kDa |
| Observed molecular weight | 35-45 kDa |
| GenBank accession number | BC064493 |
| Gene Symbol | MYOD1 |
| Gene ID (NCBI) | 4654 |
| RRID | AB_10603467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15172 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOD1 antibody 18943-1-AP | Download protocol |
| IHC protocol for MYOD1 antibody 18943-1-AP | Download protocol |
| IF protocol for MYOD1 antibody 18943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Nat Commun The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle. | ||
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. |