MYOD1 Monoclonal antibody
MYOD1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
5D1D12
Cat no : 66214-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human skeletal muscle tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
Product Information
66214-1-Ig targets MYOD1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, rat, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MYOD1 fusion protein Ag13512 相同性解析による交差性が予測される生物種 |
Full Name | myogenic differentiation 1 |
Calculated molecular weight | 320 aa, 35 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC064493 |
Gene symbol | MYOD1 |
Gene ID (NCBI) | 4654 |
RRID | AB_2881605 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)
Protocols
Product Specific Protocols | |
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WB protocol for MYOD1 antibody 66214-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) Vitellogenin 2 promotes muscle development and stimulates the browning of white fat | ||
Front Oncol Deoxypodophyllotoxin Inhibits Non-Small Cell Lung Cancer Cell Growth by Reducing HIF-1α-Mediated Glycolysis. | ||
Toxicol Appl Pharmacol A novel oleanolic acid derivative HA-19 ameliorates muscle atrophy via promoting protein synthesis and preventing protein degradation. | ||
Int J Stem Cells Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells | ||
Anim Biotechnol Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells |