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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using MYOF Recombinant antibody (82917-1-RR)

    FC experiment of HepG2 using 82917-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using MYOF Recombinant antibody (82917-1-RR)

    FC experiment of HeLa using 82917-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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MYOF Recombinant antibody

MYOF Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 82917-1-RR
Clone No.230213C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82917-1-RR targets MYOF in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myoferlin
Calculated molecular weight 235 kDa
GenBank accession numberNM_133337
Gene Symbol MYOF
Gene ID (NCBI) 26509
RRIDAB_3670651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.

Protocols

Product Specific Protocols
FC protocol for MYOF antibody 82917-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols