MYOM3 Polyclonal antibody

Name: MYOM3 Polyclonal antibody
Brand: Proteintech
SKU: 17692-1-AP

MYOM3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17692-1-AP

Synonyms

Myomesin-3, Myomesin family member 3, Myomesin 3

Validation Data Gallery

Filter:

AllWBIPIHCIF-P

WB analysis using 17692-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 17692-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 17692-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 17692-1-AP
IP result of anti-MYOM3 (IP:17692-1-AP, 4ug; Detection:17692-1-AP 1:1000) with mouse skeletal muscle tissue lysate 1280 ug.
IHC staining of human skeletal muscle using 17692-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 17692-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse skeletal muscle using 17692-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 17692-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 17692-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (17692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 17692-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human skeletal muscle tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:400
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IF	See 1 publications below
IP	See 1 publications below
ELISA	See 2 publications below

Product Information

17692-1-AP targets MYOM3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYOM3 fusion protein Ag11849 相同性解析による交差性が予測される生物種
Full Name	myomesin family, member 3
Calculated molecular weight	162 kDa, 136 kDa
Observed molecular weight	162 kDa
GenBank accession number	BC067101
Gene symbol	MYOM3
Gene ID (NCBI)	127294
RRID	AB_2146624
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)

Protocols

Product Specific Protocols
WB protocol for MYOM3 antibody 17692-1-AP	Download protocol
IHC protocol for MYOM3 antibody 17692-1-AP	Download protocol
IF protocol for MYOM3 antibody 17692-1-AP	Download protocol
IP protocol for MYOM3 antibody 17692-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	ELISA	Nat Commun An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species Authors - Ai Vu Hong View Article
mouse	WB	J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III Authors - Antoine Gardin View Article
mouse	WB	Nucleic Acids Res Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides. Authors - Karima Relizani View Article
human	WB, IP	Hum Mol Genet Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies. Authors - Jérémy Rouillon View Article
mouse	WB	Int J Mol Sci Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy. Authors - Nathalie Bourg View Article
human	IF	Stem Cell Reports A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. Authors - Matthew J Birket View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYOM3 Polyclonal antibody

MYOM3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 17692-1-AP

WB analysis of mouse skeletal muscle using 17692-1-AP

WB analysis of mouse skeletal muscle using 17692-1-AP

IP experiment of mouse skeletal muscle using 17692-1-AP

IHC staining of human skeletal muscle using 17692-1-AP

IHC staining of human skeletal muscle using 17692-1-AP

IHC staining of mouse skeletal muscle using 17692-1-AP

IHC staining of mouse skeletal muscle using 17692-1-AP

IF Staining of mouse skeletal muscle using 17692-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species

A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III

Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides.

Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies.

Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy.

A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.