Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| ELISA | See 3 publications below |
Product Information
17692-1-AP targets MYOM3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYOM3 fusion protein Ag11849 相同性解析による交差性が予測される生物種 |
| Full Name | myomesin family, member 3 |
| Calculated molecular weight | 162 kDa, 136 kDa |
| Observed molecular weight | 162 kDa |
| GenBank accession number | BC067101 |
| Gene Symbol | MYOM3 |
| Gene ID (NCBI) | 127294 |
| RRID | AB_2146624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VTT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IHC protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IF protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| IP protocol for MYOM3 antibody 17692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species | ||
J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III | ||
Nucleic Acids Res Palmitic acid conjugation enhances potency of tricyclo-DNA splice switching oligonucleotides. | ||
Hum Mol Genet Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies. | ||
Int J Mol Sci Co-Administration of Simvastatin Does Not Potentiate the Benefit of Gene Therapy in the mdx Mouse Model for Duchenne Muscular Dystrophy. | ||
Stem Cell Reports A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. |