Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
10731-1-AP targets MYOT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYOT fusion protein Ag1112 相同性解析による交差性が予測される生物種 |
| Full Name | myotilin |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55-57 kDa, 35 kDa |
| GenBank accession number | BC005376 |
| Gene Symbol | MYOT |
| Gene ID (NCBI) | 9499 |
| RRID | AB_2297956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOT antibody 10731-1-AP | Download protocol |
| IHC protocol for MYOT antibody 10731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurology Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. | ||
Neurol Genet Novel mutation in TNPO3 causes congenital limb-girdle myopathy with slow progression. | ||
Elife Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy | ||
Eur J Neurol Novel mutations in DNAJB6 cause LGMD1D and distal myopathy in French families | ||
Neurol Neuroimmunol Neuroinflamm Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy: Practical Importance of Anti-α-Actinin Immunostaining |