Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
13160-1-AP targets MYOZ1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | fish, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYOZ1 fusion protein Ag3806 相同性解析による交差性が予測される生物種 |
| Full Name | myozenin 1 |
| Calculated molecular weight | 299 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC025753 |
| Gene Symbol | MYOZ1 |
| Gene ID (NCBI) | 58529 |
| RRID | AB_2282208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NP98 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myozenin-1 is a protein that in humans is encoded by the MYOZ1 gene. MYOZ1 has been shown to interact with Telethonin, and Actinin, alpha. The MW of this protein is 32 kDa, and Catalog#13160-1-AP specially recognises the 32 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYOZ1 antibody 13160-1-AP | Download protocol |
| IP protocol for MYOZ1 antibody 13160-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Physiol NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading. | ||
J Muscle Res Cell Motil Reduced expression of MyHC slow isoform in rat soleus during unloading is accompanied by alterations of endogenous inhibitors of calcineurin/NFAT signaling pathway. | ||
J Appl Physiol (1985) Plantar mechanical stimulation prevents calcineurin/NFATc1 inactivation and slow-to-fast fiber-type shift in rat soleus muscle under hindlimb unloading. |