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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    WB analysis of Raji using 66506-1-Ig (same clone as 66506-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    WB analysis of K-562 using 66506-1-Ig (same clone as 66506-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    WB analysis of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    WB analysis of HEK-293 using 66506-1-Ig (same clone as 66506-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    WB analysis of Jurkat using 66506-1-Ig (same clone as 66506-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYPT1 Monoclonal antibody (66506-1-Ig)

    IF Staining of HeLa using 66506-1-Ig (same clone as 66506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66506-1-PBS in a different storage buffer formulation.

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MYPT1 Monoclonal antibody, PBS Only

MYPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66506-1-PBS
Clone No.2A1A9

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

MBS, MYPT1, PPP1R12A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66506-1-PBS targets MYPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen MYPT1 fusion protein Ag17496 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Calculated molecular weight 1030 aa, 115 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC111752
Gene Symbol MYPT1
Gene ID (NCBI) 4659
RRIDAB_2881869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14974
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.