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  • Featured Product
  • KD/KO Validated

MYSM1-Specific Polyclonal antibody

MYSM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20078-1-AP

Synonyms

MYSM1, KIAA1915, EC:3.4.19.-, Deubiquitinase MYSM1, 2A-DUB

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    WB analysis of A549 using 20078-1-AP

    WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    WB analysis of HeLa using 20078-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    WB analysis of HepG2 using 20078-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    WB analysis of A549 using 20078-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    WB analysis of HEK-293 using 20078-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IP experiment of HeLa using 20078-1-AP

    IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IP experiment of HeLa using 20078-1-AP

    IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:1000) with HeLa cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of mouse small intestine using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of human pancreas cancer using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of human urothelial carcinoma using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of mouse kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of mouse kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of rat kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of rat small intestine using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IHC staining of human spleen using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MYSM1-Specific Polyclonal antibody (20078-1-AP)

    IF Staining of A431 using 20078-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue, human spleen tissue, mouse small intestine tissue, human pancreas cancer tissue, human urothelial carcinoma tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20078-1-AP targets MYSM1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Myb-like, SWIRM and MPN domains 1
Calculated molecular weight 95 kDa
Observed molecular weight 81 kDa
GenBank accession numberNM_001085487
Gene symbol MYSM1
Gene ID (NCBI) 114803
RRIDAB_10664926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

Protocols

Product Specific Protocols
WB protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IHC protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IF protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IP protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Discov

MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.

Authors - Xiaolin Guan
View Article