Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 13751-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13751-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13751-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13751-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13751-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 13751-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 13751-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13751-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13751-1-AP
IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.
IHC staining of rat testis using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 13751-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 13751-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

MYST2 Polyclonal antibody

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13751-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human thyroid cancer tissue, mouse stomach tissue, rat stomach tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 3 publications below
IP	See 3 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

13751-1-AP targets MYST2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYST2 fusion protein Ag4693 相同性解析による交差性が予測される生物種
Full Name	MYST histone acetyltransferase 2
Calculated molecular weight	611 aa, 71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC032640
Gene Symbol	MYST2
Gene ID (NCBI)	11143
RRID	AB_2266703
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95251
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

Protocols

Product Specific Protocols
WB protocol for MYST2 antibody 13751-1-AP	Download protocol
IHC protocol for MYST2 antibody 13751-1-AP	Download protocol
IF protocol for MYST2 antibody 13751-1-AP	Download protocol
IP protocol for MYST2 antibody 13751-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li KO Validated View Article
human	WB	Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells Authors - Ling Li View Article
human	WB,IF,IP	Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. Authors - Guokai Yan View Article
human	WB	Cancer Res Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells. Authors - Mylinh T Duong View Article
human	WB	Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Authors - Heguo Yu View Article
human	WB	Mol Cell Proteomics Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human. Authors - Ding Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 13751-1-AP

WB analysis of Jurkat using 13751-1-AP

WB analysis of Jurkat using 13751-1-AP

WB analysis of NIH/3T3 using 13751-1-AP

WB analysis of MCF-7 using 13751-1-AP

WB analysis of PC-3 using 13751-1-AP

WB analysis of human testis using 13751-1-AP

WB analysis of HeLa using 13751-1-AP

IP experiment of HeLa using 13751-1-AP

IHC staining of rat testis using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

IHC staining of human pancreas cancer using 13751-1-AP

IHC staining of human thyroid cancer using 13751-1-AP

IHC staining of human thyroid cancer using 13751-1-AP

IHC staining of mouse stomach using 13751-1-AP

IHC staining of mouse stomach using 13751-1-AP

IHC staining of rat stomach using 13751-1-AP

IHC staining of rat stomach using 13751-1-AP

IHC staining of rat testis using 13751-1-AP

IF Staining of NIH/3T3 using 13751-1-AP

MYST2 Polyclonal antibody

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.