Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
Product Information
25234-1-AP targets MYT1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYT1L fusion protein Ag18698 相同性解析による交差性が予測される生物種 |
| Full Name | myelin transcription factor 1-like |
| Calculated molecular weight | 1186 aa, 133 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC137273 |
| Gene Symbol | MYT1L |
| Gene ID (NCBI) | 23040 |
| RRID | AB_2879978 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYT1L antibody 25234-1-AP | Download protocol |
| IHC protocol for MYT1L antibody 25234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation. | ||
Cell Prolif Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors | ||
Brain Res Bull miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L
| ||
J Biol Chem Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis | ||
Nat Neurosci Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy | ||
Genome Res MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain |