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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using MZB1 Polyclonal antibody (11454-1-AP)

    WB analysis of Raji using 11454-1-AP (same clone as 11454-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MZB1 Polyclonal antibody (11454-1-AP)

    WB analysis of Raji using 11454-1-AP (same clone as 11454-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MZB1 Polyclonal antibody (11454-1-AP)

    IHC staining of human gliomas using 11454-1-AP (same clone as 11454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11454-1-AP (MZB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MZB1 Polyclonal antibody (11454-1-AP)

    IHC staining of human small intestine using 11454-1-AP (same clone as 11454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MZB1 Polyclonal antibody (11454-1-AP)

    IHC staining of human small intestine using 11454-1-AP (same clone as 11454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11454-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11454-1-PBS targets MZB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MZB1 fusion protein Ag2015 相同性解析による交差性が予測される生物種
Full Name hypothetical protein MGC29506
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight 19-21 kDa
GenBank accession numberBC021275
Gene Symbol MZB1
Gene ID (NCBI) 51237
RRIDAB_832820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU39
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.