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Host
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse MCP-1/CCL2 Recombinant antibody, PBS Only

MCP-1/CCL2 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230483F11

Cat no : 83157-5-PBS

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using MCP-1/CCL2 Recombinant antibody (83157-5-RR)

    WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83157-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83157-5-RR

    Affinity and Kinetic Characterization of 83157-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83157-5-PBS is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
Observed molecular weight16 kDa
GenBank accession numberNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.