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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse MCP-1/CCL2 Recombinant antibody, PBS Only (Capture)

MCP-1/CCL2 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230483B2

Cat no : 83157-6-PBS

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00168-4

    Sandwich ELISA standard curve of MP00168-4

    Sandwich ELISA standard curve of MP00168-4, Mouse MCP-1 Recombinant Matched Antibody Pair - PBS only. 83157-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0427. 83157-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00168-4

    Sample test of MP00168-4

    J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 10*6 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. Cells were untreated or treated with 500 ng/mL recombinant mouse IFN-γ and 10 ng/mL LPS. An aliquot of the cell culture supernatants were removed, assayed for mouse MCP-1, and measured 6,956.6 pg/mL and 58,869.9 pg/mL.

  • Sample test of MP00168-4

    Sample test of MP00168-4

    Serum of sixteen mice was measured. The MCP-1 concentration of detected samples was determined to be 77.0 pg/mL with a range of 39.7 - 135.3 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83157-6-PBS targets MCP-1/CCL2 as part of a matched antibody pair:

MP00168-4: 83157-6-PBS capture and 83157-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
GenBank accession numberNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.