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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody, PBS Only

MCP-1/CCL2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230483A9

Cat no : 98028-1-PBS

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody (98028-1-RR)

    FC experiment of RAW 264.7 using 98028-1-RR (same clone as 98028-1-PBS)

    1x10^6 unstimulated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98028-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody (98028-1-RR)

    FC experiment of RAW 264.7 using 98028-1-RR (same clone as 98028-1-PBS)

    1x10^6 unstimulated (black) or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98028-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98028-1-RR

    Affinity and Kinetic Characterization of 98028-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98028-1 -RR against Mouse Mcp1 were performed. The affinity constant is below 1 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98028-1-PBS is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
GenBank accession numberNM-011333
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.