Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse stomach tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
51027-2-AP targets MECR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MECR fusion protein Ag0465 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial trans-2-enoyl-CoA reductase |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 37-40 kDa |
| GenBank accession number | BC003864 |
| Gene Symbol | MECR |
| Gene ID (NCBI) | 26922 |
| RRID | AB_615013 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9DCS3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MECR antibody 51027-2-AP | Download protocol |
| IF protocol for MECR antibody 51027-2-AP | Download protocol |
| IP protocol for MECR antibody 51027-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder. | ||
Elife Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. | ||
J Neurosci Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice. | ||
Hum Mol Genet Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice. | ||
Nat Commun An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration | ||
Lipids Health Dis A novel lipid metabolism-based risk model associated with immunosuppressive mechanisms in diffuse large B-cell lymphoma |