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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Methylmalonyl Coenzyme A mutase/MUT Recombinant an (84879-5-RR)

    WB analysis using 84879-5-RR (same clone as 84879-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84879-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Methylmalonyl Coenzyme A mutase/MUT Recombinant an (84879-5-RR)

    FC experiment of A431 using 84879-5-RR (same clone as 84879-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody (84879-5-RR, Clone:242198F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84879-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84879-5-RR

    Affinity and Kinetic Characterization of 84879-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84879-5-RR against Human Methylmalonyl Coenzyme A mutase/MUT were performed. The affinity constant is 1.83 nM.

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Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody, PBS Only

Methylmalonyl Coenzyme A mutase/MUT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84879-5-PBS
Clone No.242198F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

MUT, Methylmalonyl-CoA isomerase, Methylmalonyl Coenzyme A mutase, Methylmalonyl CoA isomerase, MCM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84879-5-PBS targets Methylmalonyl Coenzyme A mutase/MUT in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523 相同性解析による交差性が予測される生物種
Full Name methylmalonyl Coenzyme A mutase
Calculated molecular weight 750 aa, 83 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC016282
Gene Symbol MUT
Gene ID (NCBI) 4594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22033
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.