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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Midkine Recombinant antibody

Midkine Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241880H1

Cat no : 84815-1-RR

Synonyms

MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein, 241880H1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Midkine Recombinant antibody (84815-1-RR)

    IF Staining of HeLa using 84815-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Midkine antibody (84815-1-RR, Clone: 241880H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84815-1-RR

    Affinity and Kinetic Characterization of 84815-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84815-1-RR against Human Midkine were performed. The affinity constant is 35.9 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84815-1-RR targets Midkine in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Midkine fusion protein Ag24547 相同性解析による交差性が予測される生物種
Full Name midkine (neurite growth-promoting factor 2)
Calculated molecular weight 16 kDa
GenBank accession numberBC011704
Gene symbol Midkine
Gene ID (NCBI) 4192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

Protocols

Product Specific Protocols
IF protocol for Midkine antibody 84815-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols