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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mlkl Polyclonal antibody

Mlkl Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30145-1-AP

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Mlkl Polyclonal antibody (30145-1-AP)

    WB analysis using 30145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Mlkl Polyclonal antibody (30145-1-AP)

    WB analysis using 30145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Mlkl Polyclonal antibody (30145-1-AP)

    IF Staining of NIH/3T3 using 30145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Mlkl Polyclonal antibody (30145-1-AP)

    IF Staining of HepG2 using 30145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected in4T1 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30145-1-AP targets Mlkl in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mlkl fusion protein Ag32514 相同性解析による交差性が予測される生物種
Full Name mixed lineage kinase domain-like
Calculated molecular weight54kd
Observed molecular weight 54 kDa
GenBank accession numberNM_001310613
Gene symbol Mlkl
Gene ID (NCBI) 74568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Protocols

Product Specific Protocols
WB protocol for Mlkl antibody 30145-1-APDownload protocol
IF protocol for Mlkl antibody 30145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Metformin switches cell death modes to soothe the apical periodontitis via ZBP1

Authors - Hui Liu
View Article