Mmp9 Polyclonal antibody
Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 30592-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, mouse spleen tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
30592-1-AP targets Mmp9 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.
| Tested Reactivity | mouse, human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 9 |
| Calculated molecular weight | 81kd |
| Observed molecular weight | 80-100 kDa |
| GenBank accession number | NM_013599 |
| Gene symbol | Mmp9 |
| Gene ID (NCBI) | 17395 |
| RRID | AB_3086370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| IF protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| IP protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Lett Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells | ||
J Inflamm Res A Neutrophil Extracellular Traps-Related Gene Trait Revealed the Prospective Therapy Strategy of Coronary Atherosclerosis |
