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  • Featured Product
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Acetyl-Histone H3 (Lys9) Polyclonal antibody

Acetyl-Histone H3 (Lys9) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29133-1-AP

Synonyms

HIST1H3A, Histone-H3, HistoneH3, Histone H3, Histone H 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono-Acetyl-Histone H3 (Lys9) Polyclonal antibody (29133-1-AP)

    WB analysis using 29133-1-AP

    Untreated and Trichostatin A treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 29133-1-AP (Mono-Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Mono-Acetyl-Histone H3 (Lys9) Polyclonal antibody (29133-1-AP)

    WB analysis of HEK-293 using 29133-1-AP

    WB result of Mono-Acetyl-Histone H3 (Lys9) antibody (29133-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mono-Acetyl-Histone H3 (Lys9) transfected HEK-293 cells.

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29133-1-AP targets Acetyl-Histone H3 (Lys9) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys9) antibody 29133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Neurochem Res

The Role of Acetylation and Methylation of Rat Hippocampal Histone H3 in the Mechanism of Aluminum-Induced Neurotoxicity

Authors - Jie Gao
View Article