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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mono-Methyl-Histone H3 (Lys36) Recombinant antibody, PBS Only

Mono-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241138B6

Cat no : 82825-2-PBS

Synonyms

HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    WB analysis using 82825-2-RR (same clone as 82825-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    IF Staining of HeLa using 82825-2-RR (same clone as 82825-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys36) antibody (82825-2-RR, Clone: 241138B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    FC experiment of HeLa using 82825-2-RR (same clone as 82825-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    Dot Blot experiment of peptide using 82825-2-RR (same clone as 82825-2-PBS)

    Dot blot analysis was used to confirm the specificity of Mono-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82825-2-PBS targets Mono-Methyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.