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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mono-Methyl-Histone H3 (Lys36) Recombinant antibody

Mono-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241138B6

Cat no : 82825-2-RR

Synonyms

HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    WB analysis using 82825-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    IF Staining of HeLa using 82825-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys36) antibody (82825-2-RR, Clone: 241138B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    FC experiment of HeLa using 82825-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Dot Blot experiment of peptide using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    Dot Blot experiment of peptide using 82825-2-RR

    Dot blot analysis was used to confirm the specificity of Mono-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, THP-1 cells, K-562 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82825-2-RR targets Mono-Methyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Protocols

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RRDownload protocol
IF protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols