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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mono-Methyl-Histone H3 (Lys9) Recombinant antibody

Mono-Methyl-Histone H3 (Lys9) Recombinant Antibody for WB, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

3C23

Cat no : 82821-6-RR

Synonyms

HIST1H3A, 3C23, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono-Methyl-Histone H3 (Lys9) Recombinant antibody (82821-6-RR)

    WB analysis using 82821-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of peptide using Mono-Methyl-Histone H3 (Lys9) Recombinant antibody (82821-6-RR)

    Dot Blot experiment of peptide using 82821-6-RR

    Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, THP-1 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82821-6-RR targets Mono-Methyl-Histone H3 (Lys9) in WB, Dot Blot, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.

Protocols

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys9) antibody 82821-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols