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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse HO-1/Hmox1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00318-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP00318-3

    Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

    Sample test of MP00318-3

    Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

  • Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

    Sample test of MP00318-3

    Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

  • The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

    Sample test of MP00318-3

    The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00318-3 targets HO-1/Hmox1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse HO-1/Hmox1 Recombinant antibody, PBS Only (Capture) 83291-4-PBS (240236H1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse HO-1/Hmox1 Recombinant antibody, PBS Only (Detector) 83291-2-PBS (240236G6). 100 μg. Concentration 1 mgl/ml.

Alternative HO-1/Hmox1 matched antibody pairs: MP00318-1, MP00318-2

Matched pair protein standard: Eg0938

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83291-4-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1
GenBank accession numberNM_010442.2
Gene symbol HO-1
UniprotP14901
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83291-2-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms Hmox1, HO-1, Hmox, Hemox, heme oxygenase (decycling) 1
GenBank accession numberNM_010442.2
Gene symbol HO-1
UniprotP14901
Gene ID (NCBI) 15368
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.