Mouse Hif1a Recombinant Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Rabbit / IgG
Detection: Rabbit / IgG
Reactivity
Capture: mouse
Detection: mouse
Applications
Sandwich ELISA
Range
Sandwich ELISA: 0.156-10 ng/mL
Validation Data Gallery
Tested Applications
Sandwich ELISA
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP00056-1 targets Hif1a in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.
Range: 0.156-10 ng/mL (Sandwich ELISA);
Capture antibody: Mouse Hif-1 alpha Recombinant antibody, PBS Only (Capture/Detector) 82989-3-PBS (230101H5). 100 μg. Concentration 1 mgl/ml.
Detection antibody: Mouse Hif-1 alpha Recombinant antibody, PBS Only (Detector) 82989-2-PBS (230101D11). 100 μg. Concentration 1 mgl/ml.
Alternative Hif1a matched antibody pairs: MP00056-2
Matched pair protein standard: Ag33620
Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 82989-3-PBS |
| Tested Reactivity | mouse |
| Immunogen | Hif-1 alpha fusion protein Ag33620 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | hypoxia inducible factor 1, alpha subunit |
| Synonyms | Hif1a, 230101H5, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha |
| GenBank accession number | NM_001313919 |
| Gene symbol | Hif1a |
| Uniprot | Q61221 |
| Gene ID (NCBI) | 15251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 82989-2-PBS |
| Tested Reactivity | mouse |
| Immunogen | Hif-1 alpha fusion protein Ag33620 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | hypoxia inducible factor 1, alpha subunit |
| Synonyms | Hif1a, 230101D11, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha |
| GenBank accession number | NM_001313919 |
| Gene symbol | Hif1a |
| Uniprot | Q61221 |
| Gene ID (NCBI) | 15251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |